Biotinylated Human MDL-1/CLEC5A Protein
Catalog No: MDL1-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human MDL-1/CLEC5A Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Pro28-Lys188 (UniProt accession: Q9NY25-1).
Product Details
- Molecular Aliases
- MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
- Protein Length
- Pro28-Lys188
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MDL-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human MDL-1, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. (QC Test)
Background
In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor. MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
References
- Riesen M, Feyst I, Rattanavirotkul N, Ezcurra M, Tullet JM, Papatheodorou I, Ziehm M, Au C, Gilliat AF, Hellberg J, Thornton JM, Gems D. MDL-1, a growth- and tumor-suppressor, slows aging and prevents germline hyperplasia and hypertrophy in C. elegans. Aging (Albany NY). 2014 Feb;6(2):98-117. doi: 10.18632/aging.100638. PMID: 24531613; PMCID: PMC3969279.
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