Biotinylated Human IL-23R Protein (Primary Amine Labeling)
Catalog No: IL23R-HF002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-23R Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly24-Gly355 (UniProt accession: Q5VWK5-1).
Product Details
- Molecular Aliases
- IBD17; IL-23 R; IL-23 receptor; IL23R; IL23 receptor
- Protein Length
- Gly24-Gly355
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 64.81 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human IL-23R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human IL-23R, hFc Avi tag captured on CM5 Chip via Protein A can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 42.04 nM as determined in SPR assay (Biacore T200).
Background
Interleukin-23 (IL-23), a member of the IL-12 family of cytokines, is a heterodimeric cytokine. It is composed of subunits p40 (shared with IL-12) and p19 (an IL-12 p35-related subunit) and is secreted by several types of immune cells, such as natural killer cells and dendritic cells. The IL-23 receptor is composed of the subunit IL-12Rβ1 and the IL-23-specific subunit IL-23R. The binding of IL-23 to its specific cell surface receptor regulates a number of functions, including proliferation and differentiation of cells and secretion of cell factors.
References
- Li Y, Wang H, Lu H, Hua S. Regulation of Memory T Cells by Interleukin-23. Int Arch Allergy Immunol. 2016;169(3):157-62. doi: 10.1159/000445834. Epub 2016 Apr 22. PMID: 27100864.
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