Human IL-23R Protein
Catalog No: IL23R-HF003
- Species
- Human
- Expression System
- HEK293
- Tag
- an mFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-23R Protein is expressed in HEK293 cells with an mFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly24-Gly355 (UniProt accession: Q5VWK5-1).
Product Details
- Molecular Aliases
- IBD17; IL-23 R; IL-23 receptor; IL23R; IL23 receptor
- Protein Length
- Gly24-Gly355
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 10% trehalose (pH 7.4).
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-23R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-23R is greater than 85% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 36.4ng/ml determined by ELISA (QC Test).
Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His-Avi tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200).
Background
Interleukin-23 (IL-23), a member of the IL-12 family of cytokines, is a heterodimeric cytokine. It is composed of subunits p40 (shared with IL-12) and p19 (an IL-12 p35-related subunit) and is secreted by several types of immune cells, such as natural killer cells and dendritic cells. The IL-23 receptor is composed of the subunit IL-12Rβ1 and the IL-23-specific subunit IL-23R. The binding of IL-23 to its specific cell surface receptor regulates a number of functions, including proliferation and differentiation of cells and secretion of cell factors.
References
- Li Y, Wang H, Lu H, Hua S. Regulation of Memory T Cells by Interleukin-23. Int Arch Allergy Immunol. 2016;169(3):157-62. doi: 10.1159/000445834. Epub 2016 Apr 22. PMID: 27100864.
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