Biotinylated Human IL-7 R alpha/CD127 Protein
Catalog No: IL7-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-7 R alpha/CD127 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu21-Asp239 (UniProt accession: P16871-1).
Product Details
- Molecular Aliases
- IL-7R alpha; IL7Ra; CD127; CDW127; IL7Rα;IL7R alpha
- Protein Length
- Glu21-Asp239
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 28.16 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-7 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-7 R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-7R alpha, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Lusvertikimab, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. (QC Test)
Biotinylated Human IL-7R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 7.37 nM as determined in SPR assay (Biacore T200).
Background
Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
References
- Oliveira ML, Akkapeddi P, Ribeiro D, Melão A, Barata JT. IL-7R-mediated signaling in T-cell acute lymphoblastic leukemia: An update. Adv Biol Regul. 2019 Jan;71:88-96. doi: 10.1016/j.jbior.2018.09.012. Epub 2018 Sep 19. PMID: 30249539; PMCID: PMC6386770.D
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