Human APRIL/TNFSF13 Trimer Protein
Catalog No: APRIL-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Flag
- Activity
- Activity verified
Product overview
Recombinant Human APRIL/TNFSF13 Trimer Protein is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Lys112-Leu250 trimer design (UniProt accession: O75888-1).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
- Protein Length
- Lys112-Leu250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human APRIL Trimer is greater than 95% as determined by SEC-HPLC.
Immobilized Human APRIL Trimer, His Tag at 0.1 μg/ml (100 μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 0.9 ng/ml determined by ELISA (QC Test).
Immobilized Human APRIL Trimer, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-APRIL Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His-Flag Tag with an affinity constant of 0.76nM as determined in BLI assay.
Loaded Human BCMA, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His Tag with an affinity constant of 0.24 nM as determined in BLI assay.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
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