Biotinylated Human CD36/SR-B3 Protein
Catalog No: CD36-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD36/SR-B3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly30-Asn439 (UniProt accession: P16671-1).
Product Details
- Molecular Aliases
- CD36; FAT; FATCHDS7; GPIIIb; GPIV; PAS IV; PAS-4; SCARB3; SRB3; SR-B3; BDPLT10; CHDS7; GP3B; PAS4
- Protein Length
- Gly30-Asn439
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD36 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD36 is greater than 85% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD36, His Ai Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD36 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. (QC Test)
Background
CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions. CD36 is a multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides.
References
- Cho, Sunghee. CD36 as a Therapeutic Target for Endothelial Dysfunction in Stroke[J]. Current Pharmaceutical Design, 2012, 18(25):3721-3730.
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