Biotinylated Cynomolgus EPHA2 Protein
Catalog No: EPHA2-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Cynomolgus EPHA2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ala24-Ser534 (UniProt accession: Q1KL86).
Product Details
- Molecular Aliases
- Ephrin type-A receptor 2; EPHA2
- Protein Length
- Ala24-Ser534
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus EPHA2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus EPHA2 is greater than 95% as determined by SEC-HPLC.
Background
Ephrin type-A receptor 2 (EPHA2) is a receptor tyrosine kinase (RTK), whose over-expression has been observed in a variety of cancers, including breast cancer. EPHA2 expression may be causally related to tumorigenesis; therefore, it is important to understand how EPHA2 gene (EPHA2) expression is regulated.
References
- Okuyama T, Sakamoto R, Kumagai K, Nishizawa M, Kimura T, Sugie T, Kimura T. EPHA2 antisense RNA modulates EPHA2 mRNA levels in basal-like/triple-negative breast cancer cells. Biochimie. 2020 Dec;179:169-180. doi: 10.1016/j.biochi.2020.10.002. Epub 2020 Oct 3. PMID: 33022313.
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