Human EPHA2 Protein
Catalog No: EPHA2-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Human EPHA2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ala24-Val537 (UniProt accession: P29317-1).
Product Details
- Molecular Aliases
- Ephrin type-A receptor 2; Epithelial cell kinase; ECK; MPK-5; MPK5; SEK-2; SEK2; Myk2; AW54528
- Protein Length
- Ala24-Val537
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 82.40 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human EPHA2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EPHA2 is greater than 95% as determined by SEC-HPLC.
Background
Erythropoietin-producing hepatocellular receptor A2 (EphA2) receptor tyrosine kinase plays an important role in tissue organization and homeostasis in normal organs. EphA2 is overexpressed in a variety of types of solid tumours with oncogenic functions.
References
- Huang C, Chen Z, He Y, He Z, Ban Z, Zhu Y, Ding L, Yang C, Jeong JH, Yuan W, Yang L. EphA2 promotes tumorigenicity of cervical cancer by up-regulating CDK6. J Cell Mol Med. 2021 Mar;25(6):2967-2975. doi: 10.1111/jcmm.16337. Epub 2021 Feb 14. PMID: 33586348; PMCID: PMC7957165.
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