Biotinylated Human CD8 alpha&beta Heterodimer Protein
Catalog No: CD8-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, a Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD8 alpha&beta Heterodimer Protein is expressed as a heterodimer of CD8 alpha and CD8 beta by co-expression in HEK293. Subunit CD8 alpha is fused with a His tag and a Avi tag at the C-terminus and subunit CD8 beta is fused with a Flag tag at the C-terminus. It contains amino acid residues Ser22-Asp182 (CD8 alpha) acidic tail and Leu22-Pro170 (CD8 beta) basic tail (accession: P01732-1(CD8 alpha) & P10966-1(CD8 beta)).
Product Details
- Molecular Aliases
- CD8 alphaβ CD8A&CD8B;
- Protein Length
- Ser22-Asp182
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 25.35 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-36 kDa and 38-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC.
The purity of Biotinylated Human CD8 alpha&beta is greater than 95% and the molecular weight of this protein is around 52-65 kDa as determined by SEC-MALS.
Immobilized Biotinylated Human CD8 alpha&beta, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD8 alpha&beta Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human CD8 alpha&beta, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Immobilized Biotinylated Human CD8 alpha&beta, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Background
CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8 + peripheral T cells bearing TCR.
References
- Nomura A, Taniuchi I. The Role of CD8 Downregulation during Thymocyte Differentiation. Trends Immunol. 2020 Nov;41(11):972-981.
- Liu Y, Cuendet MA, Goffin L, Šachl R, Cebecauer M, Cariolato L, Guillaume P, Reichenbach P, Irving M, Coukos G, Luescher IF. CD8 Binding of MHC-Peptide Complexes in cis or trans Regulates CD8 + T-cell Responses. J Mol Biol. 2019 Dec 6;431(24):4941-4958.
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