Human NKG2D/CD314 Protein
Catalog No: CD314-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Flag
- Activity
- Activity verified
Product overview
Recombinant Human NKG2D/CD314 Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Flag tag at the N-terminus. It contains amino acid residues Phe78-Val216 (UniProt accession: P26718).
Product Details
- Molecular Aliases
- CD314; D12S2489E; KLR; NKG2-D; NKG2D
- Protein Length
- Phe78-Val216
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC.
Immobilized Human MICA, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81μg/ml determined by ELISA (QC Test).
Immobilized Human MICB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.29μg/ml determined by ELISA
Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag: Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
Loaded Human NKG2D, hFc Tag on ProA-Biosensor can bind Cynomolgus ULBP-2, His Tag (Cat. ULB-CM102) with an affinity constant of 30.00 nM as determined in BLI assay.
Background
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
References
- Spear P, Barber A, Rynda-Apple A, et al. NKG2D CAR T-cell therapy inhibits the growth of NKG2D ligand heterogeneous tumors[J]. Immunology and Cell Biology, 2013, 91(6):435-440. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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