Human GCGR/Glucagon receptor VLP Protein
Catalog No: GCGR-HP001
- Species
- Human
- Expression System
- HEK293
Product overview
Human GCGR/Glucagon receptor VLP Protein is a full-length multi-pass membrane protein displayed in a VLP format for antibody discovery, binding assays and method development. Recombinant Human GCGR/Glucagon receptor VLP is expressed from HEK293. It contains Met1-Phe477 (It may have cross reaction with anti-His antibody) (accession: P47871).
Product Details
- Molecular Aliases
- Glucagon receptor; GL-R; GCGR; Glucagon R
- Protein Length
- Met1-Phe477
- Expression System
- HEK293
- Theoretical Molecular Weight
- The target protein has a predicted MW of 56 kDa.
- Purity
- > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in PBS, 300mM L-arginine (pH 7.4). Notice: If you need it for immunization, water-soluble adjuvant is recommended.
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Aliquot where appropriate and minimize freeze-thaw cycles.
Background
The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.
References
- Kaur S, Sokrat B, Capozzi ME, El K, Bai Y, Jazic A, Han B, Krishna Kumar K, D'Alessio DA, Campbell JE, Bouvier M, Shenoy SK. The ubiquitination status of the glucagon receptor determines signal bias. J Biol Chem. 2023 May;299(5):104690. doi: 10.1016/j.jbc.2023.104690. Epub 2023 Apr 8. PMID: 37037304; PMCID: PMC10200972.
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