Biotinylated Human GCGR/Glucagon receptor Nanodisc Protein
Catalog No: GCGR-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
Product overview
Biotinylated Human GCGR/Glucagon receptor Nanodisc Protein is a full-length multi-pass membrane protein presented in a Nanodisc format for antibody discovery, binding assays and method development. Recombinant Biotinylated Human GCGR/Glucagon receptor Nanodisc is expressed from HEK293 with His tag at the C-terminus. It contains Met1-Phe477 (accession: P47871).
Product Details
- Molecular Aliases
- Glucagon receptor; GL-R; GCGR; Glucagon R
- Protein Length
- Met1-Phe477
- Expression System
- HEK293
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Aliquot where appropriate and minimize freeze-thaw cycles.
Background
The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.
References
- Kaur S, Sokrat B, Capozzi ME, El K, Bai Y, Jazic A, Han B, Krishna Kumar K, D'Alessio DA, Campbell JE, Bouvier M, Shenoy SK. The ubiquitination status of the glucagon receptor determines signal bias. J Biol Chem. 2023 May;299(5):104690. doi: 10.1016/j.jbc.2023.104690. Epub 2023 Apr 8. PMID: 37037304; PMCID: PMC10200972.
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