Biotinylated Human CD155/PVR Protein
Catalog No: CD155-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD155/PVR Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Trp21-Asn343 (UniProt accession: P15151-1).
Product Details
- Molecular Aliases
- CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
- Protein Length
- Trp21-Asn343
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD155 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag (Cat. TIG-HM210) with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag (Cat. DAM-HM201) with the EC50 of 10.6ng/ml determined by ELISA.
Loaded Human TIGIT, hFc Tag (Cat. TIG-HM210) on Anti-Human lgG Fc Gen II (HFc II) -Biosensor can bind Biotinylated Human CD155, His-Avi tag with an affinity constant of 0.72 nM as determined in BLI assay.
Loaded Human DNAM-1, hFc Tag (Cat. DAM-HM201) on Anti-Human lgG Fc Gen II (HFc II) -Biosensor can bind Biotinylated Human CD155, His-Avi tag with an affinity constant of 0.75 nM as determined in BLI assay.
Background
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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