Mouse CD155/PVR Protein
Catalog No: CD155-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse CD155/PVR Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Asp29-Leu348 (UniProt accession: Q8K094-1).
Product Details
- Molecular Aliases
- CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
- Protein Length
- Asp29-Leu348
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse CD155, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test).
Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 μM as determined in SPR assay (Biacore T200).
Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay.
Background
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
References
- Yu X, Harden K, C Gonzalez L, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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