Biotinylated Human APRIL/TNFSF13 Trimer Protein
Catalog No: APRIL-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His , Avi, Flag
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed in HEK293 cells with a His tag, Avi tag and Flag Tag at the N-terminus. It contains amino acid residues Lys112-Leu250 trimer design (UniProt accession: O75888-1).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
- Protein Length
- Lys112-Leu250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human APRIL Trimer is greater than 90% as determined by SEC-HPLC.
Immobilized Human BCMA, hFc Tag (Cat. BCM-HM217) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer), His Tag with the EC50 of 11.9ng/ml determined by ELISA.
Immobilized Biotinylated Human APRIL (Trimer), His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TACI, hFc Tag (Cat. TAC-HM213) with the EC50 of 7.3ng/ml determined by ELISA.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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