Human APRIL/TNFSF13 Protein (active trimer)
Catalog No: APRIL-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Flag
- Activity
- Activity verified
Product overview
Recombinant Human APRIL/TNFSF13 Protein is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Ala105-Leu250 (UniProt accession: O75888-1).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
- Protein Length
- Ala105-Leu250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.16 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human APRIL is greater than 95% as determined by SEC-HPLC.
The purity of Human APRIL is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.
Immobilized Human APRIL, His Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag (Cat. BCM-HM217) with the EC50 of 1.2ng/ml determined by ELISA. (QC Test)
Immobilized Human APRIL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TACI, hFc Tag (Cat. TAC-HM213) with the EC50 of 21.1ng/ml determined by ELISA.
Immobilized Human APRIL, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-APRIL Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
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