Human/Mouse/Rat Activin A Protein
Catalog No: ACTIVINA-HP001
- Species
- Human
- Expression System
- HEK293
- Activity
- Activity verified
Product overview
Recombinant Human/Mouse/Rat Activin A Protein is expressed in HEK293 cells without tag. It contains amino acid residues Gly311-Ser426 (UniProt accession: P08476).
Product Details
- Molecular Aliases
- INHBA; Activin A; Inhibin beta A chain;
- Protein Length
- Gly311-Ser426
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
Immobilized Human/Mouse/Rat Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag (Cat. ARB-HM22B) with the EC50 of 3.7ng/ml determined by ELISA. (QC Test)
Immobilized Human/Mouse/Rat Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Immobilized Human/Mouse/Rat Activin A, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Garetosmab, hFc Tag with the EC50 of 61.2ng/ml determined by ELISA.
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200).
Background
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
References
- PMID: 31416681.
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