Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein
Catalog No: PRAME-HP005
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide (accession: A0A140T913 (HLA-A*02:01) & P61769 (B2M) &SLLQHLIGL).
Product Details
- Molecular Aliases
- PRAME; OIP-4; OIP4; MAPE;
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA. (QC Test)
Loaded HLA-A*02:01&B2M&PRAME TCR, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer with an affinity constant of 48.80 pM as determined in BLI assay.
Background
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
References
- Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.
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