Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein
Catalog No: PRAME-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &SLLQHLIGL).
Product Details
- Molecular Aliases
- PRAME; OIP-4; OIP4; MAPE;
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 51-58 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer (Cat. No.MHC-HM443B) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.
Background
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
References
- Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.
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