Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein
Catalog No: APRIL-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His , Flag, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed in HEK293 cells with a His tag, Flag tag and Avi tag at the N-terminus. It contains amino acid residues Lys112-Leu250 trimer design (UniProt accession: A0A2K5TJA1).
Product Details
- Molecular Aliases
- CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
- Protein Length
- Lys112-Leu250
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus APRIL Trimer is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Cynomolgus APRIL (Trimer), His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-APRIL Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Background
The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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