Human RANKL/TNFSF11/CD254 Protein
Catalog No: RANKL-HP001
- Species
- Human
- Expression System
- HEK293
- Activity
- Activity verified
Product overview
Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed in HEK293 cells without tag. It contains amino acid residues Gly63-Asp244 (UniProt accession: O14788-2).
Product Details
- Molecular Aliases
- ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
- Protein Length
- Gly63-Asp244
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Bis-Tris PAGE resu
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human RANKL is greater than 95% as determined by SEC-HPLC.
Immobilized Human RANKL, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test).
Immobilized Human RANKL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.2ng/ml determined by ELISA.
Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Background
Receptor activator of nuclear factor κB (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
References
- Nagy V, Penninger JM. The RANKL-RANK Story. Gerontology. 2015;61(6):534-42. doi: 10.1159/000371845. Epub 2015 Feb
- PMID: 25720990.
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