Mouse OX40 Ligand/TNFSF4 Protein
Catalog No: OX40-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Mouse OX40 Ligand/TNFSF4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ser51-Leu198 (UniProt accession: P43488).
Product Details
- Molecular Aliases
- OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34
- Protein Length
- Ser51-Leu198
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 52-56 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse OX40 Ligand is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse OX-40 Ligand, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 0.91μg/ml determined by ELISA. (QC Test)
Background
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
References
- Al-Shamkhani A, Mallett S, Brown M H, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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