Biotinylated Human ACE2/ACEH Protein
Catalog No: ACE2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ACE2/ACEH Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln18-Ser740 (UniProt accession: Q9BYF1-1).
Product Details
- Molecular Aliases
- ACE-2; ACEH; ACE2
- Protein Length
- Gln18-Ser740
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-CoV-2 Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 40.6ng/ml determined by ELISA (QC Test).
Background
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
References
- Kuba K, Imai Y, Rao S, et al. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus–induced lung injury[J]. Nature Medicine, 2005, 11(8):875-879. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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