Biotinylated Human ACE2/ACEH Protein
Catalog No: ACE2-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ACE2/ACEH Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Gln18-Ser740 (UniProt accession: Q9BYF1-1).
Product Details
- Molecular Aliases
- ACE-2; ACEH; ACE2
- Protein Length
- Gln18-Ser740
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized SARS-CoV-2 Spike S, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
Loaded Biotinylated Human ACE2, hFc-Avi tag on Streptavidin-Biosensor can bind SARS-CoV-2 Spike S, hFc-Avi tag with an affinity constant of 0.17 µM as determined in BLI assay.
Background
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
References
- Kuba K, Imai Y, Rao S, et al. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus–induced lung injury[J]. Nature Medicine, 2005, 11(8):875-879.
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