Human IL-7 Protein
Catalog No: IL7-HP001
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant Human IL-7 Protein is expressed in E. coli without tag. It contains amino acid residues Asp26-His177 (UniProt accession: P13232).
Product Details
- Molecular Aliases
- IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
- Protein Length
- Asp26-His177
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.100-1 ng/mL. (QC Test)
Immobilized Human IL-7 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC tested).
Human IL-7R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7, No Tag with an affinity constant of 35.38 nM as determined in SPR assay (Biacore S200).
Background
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
References
- Park JH, Waickman AT, Reynolds J, Castro M, Molina-París C. IL7 receptor signaling in T cells: A mathematical modeling perspective. Wiley Interdiscip Rev Syst Biol Med. 2019;11(5):e1447. doi:10.1002/wsbm.1447
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