Non-biotinylated Human OX40/TNFRSF4/CD134 Protein
Catalog No: OX40-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu29-Ala216 (UniProt accession: P43489).
Product Details
- Molecular Aliases
- CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue; TXGP1L; IMD16
- Protein Length
- Leu29-Ala216
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human OX40 is greater than 95% as determined by SEC HPLC.
Immobilized Human OX40, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Human OX40, His-Avi tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
References
- Al-Shamkhani A, Mallett S, Brown M H, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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