Human HVEM/TNFRSF14 Protein
Catalog No: HVEM-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human HVEM/TNFRSF14 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Leu39-Val202 (UniProt accession: Q92956-1).
Product Details
- Molecular Aliases
- CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2
- Protein Length
- Leu39-Val202
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human HVEM, His Tag at 5μg/ml (100 μl/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 84.6 ng/ml determined by ELISA (QC Test).
Immobilized Human HVEM, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA.
Immobilized Human HVEM, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Human LIGHT, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA.
Loaded Human HVEM, His Tag on Anti-His-Biosensor can bind Human BTLA, hFc (Cat. BTL-HM201) Tag with an affinity constant of 3.67 nM as determined in BLI assay.
Loaded Human HVEM, His Tag on Anti-His-Biosensor can bind Human CD160, hFc Tag (Cat. CD1-HM260) with an affinity constant of 42.80 nM as determined in BLI assay.
Background
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
References
- Granger S W, Rickert S. LIGHT-HVEM signaling and the regulation of T cell-mediated immunity[J]. Cytokine Growth Factor Rev, 2003, 14(3):289-296.
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