Human HVEM/TNFRSF14 Protein
Catalog No: HVEM-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human HVEM/TNFRSF14 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu39-Val202 (UniProt accession: Q92956-1).
Product Details
- Molecular Aliases
- CD270; CD40-like protein; HVEM; LIGHTR; ATAR; TNFRSF14; TR2
- Protein Length
- Leu39-Val202
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human HVEM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human LIGHT, His Tag (Cat. LGT-HM131) at 5μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. (QC Test)
Immobilized Human BTLA, His Tag (Cat. BTL-HM401) at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 11.5 ng/ml determined by ELISA.
Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 μM as determined in SPR assay (Biacore T200).
Background
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
References
- Granger S W, Rickert S. LIGHT-HVEM signaling and the regulation of T cell-mediated immunity[J]. Cytokine Growth Factor Rev, 2003, 14(3):289-296.
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