PROVETOP

Biotinylated Proteins

Site-specific and chemically biotinylated recombinant proteins for binding analysis, capture assays and screening workflows.

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Overview

PROVETOP biotinylated proteins support streptavidin-based capture, SPR and BLI analysis, antibody screening and other binding workflows. Select the target, species and tag format that fit the intended assay, then review product-specific activity data before use.

Flexible labeling formats

Compare Avi-tag and biotinylated constructs by target and intended workflow.

Assay-oriented selection

Filter by species and tag to narrow the catalog for capture or binding studies.

Product-specific evidence

Review published binding curves and affinity results for the named construct.

Choose by assay

Start with your experimental goal, then see the recommended biotinylation approach for reliable results.

Illustrated assay route from SPR and BLI through ELISA screening to streptavidin capture

SPR & BLI

Real-time binding analysis of molecular interactions.

Avi-tag preferred

Site-specific labeling supports controlled protein orientation on streptavidin surfaces.

ELISA & screening

High-throughput assays for detection and screening.

Either format

Both Avi-tag and primary-amine labeling work well for immunoassays and screening.

Streptavidin capture

Pull-down and immobilization for purification or enrichment.

Avi-tag preferred

Site-specific labeling enables consistent orientation and strong capture on streptavidin.

Biotinylated Protein Products

1 product

Performance

Activin A binding activity by ELISA

ELISA binding curve for biotinylated Activin A

Activin RIIB was coated at 1 μg/mL (100 μL/well) for the binding assay.

Reported EC50: 3.2 ng/mL (QC test)

Activin A binding affinity by BLI

BLI sensorgram for biotinylated Activin A

Biotinylated Activin A loaded on a streptavidin biosensor binds Human Activin RIIB.

Reported affinity constant: 30.40 nM

PVRIG binding activity by ELISA

ELISA binding curve for biotinylated Mouse PVRIG

Mouse Nectin-2, His tag was immobilized at 2 μg/mL (100 μL/well).

Reported EC50: 5.04 μg/mL (QC test)

PVRIG binding affinity by SPR

SPR sensorgram for biotinylated Mouse PVRIG

Biotinylated Mouse PVRIG, hFc-Avi tag captured on a CM5 chip binds Mouse Nectin-2, His tag.

Reported affinity constant: 2.22 μM (Biacore T200)

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