PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer Protein
Catalog No: H2KB-HP003
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide (accession: P01901(H-2K(b)) & P01887(B2M) &SIINFEKL).
Product Details
- Molecular Aliases
- H-2K; OVA; MHC; H2-K1;
- Protein Length
- His24-Pro297
- Expression System
- HEK293
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 0.2% BSA (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer can specifically bind to CD8+, OVA-specific T cells from OT-I TCR transgenic mice. Cells were incubated with 10 μg/ml PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer.
Background
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
References
- Kamalov M, Kählig H, Rentenberger C, Müllner ARM, Peterlik H, Becker CFW. Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel. Sci Rep. 2019 Feb 25;9(1):2696. doi: 10.1038/s41598-019-39148-8. PMID: 30804439; PMCID: PMC6390181.
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