PE-Labeled Human Peptide Ready HLA-B*27:05&B2M Tetramer Protein
Catalog No: HLAB2705-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant PE-Labeled Human Peptide Ready HLA-B*27:05&B2M Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-Labeled Human Peptide Ready HLA-B*27:05&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidin. It contains Gly25-Thr305 (HLA-B*27:05) and Ile21-Met119 (B2M) (accession: AAA36221.1(HLA-B*27:05) & P61769(B2M)).
Product Details
- Molecular Aliases
- MHC; HLA-B*27:05; Peptide Ready
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 500 kDa.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 100mM L-Arginine, 0.2% BSA (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized PE-Labeled Human Peptide Ready HLA-B*27:05&B2M Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. (QC Test)
Background
HLA-B*27:05&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-B*27:05. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
References
- [1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
- [2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.
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