PE-Labeled Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein
Catalog No: HLAG-HP006
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant PE-Labeled Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-Labeled Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide (accession: P17693-1(HLA-G) & P61769(B2M) &RIIPRHLQL).
Product Details
- Molecular Aliases
- HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in PBS, 200mM L-Arginine, 0.2% BSA (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized PE-Labeled Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.
Background
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
References
- (1) Krijgsman D, Roelands J, Hendrickx W, Bedognetti D, Kuppen PJK. HLA-G: A New Immune Checkpoint in Cancer? Int J Mol Sci. 2020 Jun 25;21(12):4528. doi: 10.3390/ijms21124528. PMID: 32630545; PMCID: PMC7350262.
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