PE-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein
Catalog No: KRASG12V-HP012
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant PE-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. PE-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and VVVGAVGVGK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &VVVGAVGVGK).
Product Details
- Molecular Aliases
- MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl, 0.2% BSA (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
1E6 of KRAS G12V specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein (Cat. No. MHC-HE005TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. PE signal was used to evaluate the binding activity. (QC Test)
Background
Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
References
- (1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.
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