PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein
Catalog No: P53R175H-HP005
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. PE-labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &HMTEVVRHC).
Product Details
- Molecular Aliases
- MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20 mM PB, 500 mM NaCl, 0.2% BSA (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
Background
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
References
- (1) Kanapathipillai M. Treating p53 Mutant Aggregation-Associated Cancer. Cancers (Basel). 2018 May 23;10(6):154. doi: 10.3390/cancers10060154. PMID: 29789497; PMCID: PMC6025594.
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