Non-biotinylated Human SIRP Beta 1 isoform 3 Protein, Ultra Low Endotoxin
Catalog No: SIRPB-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu30-Leu371 (UniProt accession: Q5TFQ8-1).
Product Details
- Molecular Aliases
- SIRP-beta-1 isoform 3; SIRPB1; CD172b; DKFZp686A05192; FLJ26614; 9930027N05Rik; SIRP-beta; Sirpb; Sirpb1; Sirpb1a;SIRP-β-1 isoform 3; SIRP-β
- Protein Length
- Glu30-Leu371
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human SIRP Beta 1 isoform 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SIRP Beta 1 isoform 3 is greater than 95% as determined by SEC-HPLC.
Background
Signal regulatory protein β1 (SIRPB1) is a signal regulatory protein member of the immunoglobulin superfamily and is capable of modulating receptor tyrosine kinase-coupled signaling. Copy number variations at the SIRPB1 locus were previously reported to associate with prostate cancer aggressiveness in patients.SIRPB1 gene amplification and overexpression were detected in prostate cancer specimens. The knockdown of SIRPB1 significantly suppressed cell growth in colony formation assays and cell mobility.
References
- Song Q, Qin S, Pascal LE, Zou C, Wang W, Tong H, Zhang J, Catalona WJ, Dhir R, Morrell M, Balasubramani GK, Lu Y, Wang Z. SIRPB1 promotes prostate cancer cell proliferation via Akt activation. Prostate. 2020 Mar;80(4):352-364. doi: 10.1002/pros.23950. Epub 2020 Jan
- PMID: 31905248; PMCID: PMC7421598. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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