Non-biotinylated Human FGFR3 beta (IIIc) Protein
Catalog No: FGFR3-HP004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human FGFR3 beta (IIIc) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp127-Gly375 (UniProt accession: P22607-1).
Product Details
- Molecular Aliases
- ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4
- Protein Length
- Asp127-Gly375
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGFR3 beta (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human FGFR3 beta (IIIc) is greater than 95% as determined by SEC-HPLC.
Immobilized Human FGFR3 beta (IIIc) at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).
Background
Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts.
References
- Hadari Y, Schlessinger J. FGFR3-targeted mAb therapy for bladder cancer and multiple myeloma[J]. The Journal of clinical investigation, 2009, 119(5):1077-1079.
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