Non-biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein
Catalog No: CD16B-HP002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly17-Ser200(NA2). The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106. The CD16b NA1 form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36,S65, N82, and I106 (UniProt accession: O75015-1).
Product Details
- Molecular Aliases
- CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; FcγRIIIB; Fc γRIIIB; Fcγ RIIIB; Fc γ RIIIB
- Protein Length
- Gly17-Ser200
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human Fc gamma RIIIB (NA2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC.
Loaded Human Fc gamma RIIIB (NA2), His Tag on Anti-His-Biosensor, can bind Rituximab, hFc Tag with an affinity constant of 2.87 µM as determined in BLI assay (Gator). (QC Test)
Human Fc gamma RIIIB (NA2), His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 2.33 μM as determined in SPR assay (Biacore T200).
Background
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
References
- Qiu W Q, Bruin D D, Brownstein B H, et al. Organization of the human and mouse low-affinity Fc gamma R genes: duplication and recombination[J]. Science, 248.
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