Non-biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein, Ultra Low Endotoxin
Catalog No: CD28-HP004
- Species
- Human/Cyno/Rhesus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Asn19-Pro152 (UniProt accession: P10747-1).
Product Details
- Molecular Aliases
- CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
- Protein Length
- Asn19-Pro152
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus/Rhesus macaque CD28 is greater than 90% as determined by SEC-HPLC.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test).
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi tag with an affinity constant of 43.61 μM as determined in SPR assay (Biacore T200).
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15 nM as determined in a SPR assay (Biacore T200).
Background
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
References
- Suntharalingam G, Perry M R, Ward S, et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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