Mouse tPA Protein
Catalog No: TPA-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Mouse tPA Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559) (UniProt accession: P11214).
Product Details
- Molecular Aliases
- t-PA; tPA; Alteplase; Reteplase; PLAT;
- Protein Length
- Ser33-Gln559
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 85.00 kDa. It migrates to 35-40 kDa and 30-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse tPA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
References
- Grummisch JA, Jadavji NM, Smith PD. tPA promotes cortical neuron survival via mTOR-dependent mechanisms. Mol Cell Neurosci. 2016 Jul;74:25-33. doi: 10.1016/j.mcn.2016.03.005. Epub 2016 Mar 16. PMID: 26995507.
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