Mouse FcRn Protein
Catalog No: FCRN-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse FcRn Protein is expressed in HEK293 cells with a His tag at the C-terminus of FCGRT. It contains amino acid residues Ser22-Ser297 (FCGRT) and Ile21-Met119 (B2M) (accession: Q61559(FCGRT) & P01887(B2M)).
Product Details
- Molecular Aliases
- FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
- Protein Length
- Ser22-Ser297
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 32.1 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 50-55 kDa (FCGRT)&14-15 kDa (B2M) based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse FcRn is greater than 95% as determined by SEC-HPLC.
Loaded Mouse FcRn, His Tag on Anti-His-Biosensor can bind Trastuzumab, hFc Tag with an affinity constant of 40.00 nM as determined in BLI assay. (QC Test)
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.76 nM as determined in SPR assay (Biacore T200).
Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 20.82 nM as determined in SPR assay (Biacore T200).
Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
Background
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells. FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14, and forms an MHC class I-like heterodimer.
References
- Kiessling P, Lledo-Garcia R, Watanabe S, et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study[J]. Science translational medicine, 2017, 9(414):eaan1208.
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