Mouse Fc gamma RIIB/CD32b Protein
Catalog No: CD32B-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Thr40-Arg217 (accession: NP_001070657.1).
Product Details
- Molecular Aliases
- Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2; FcGR; FCGR2; FCGR21; FCGR2A; FcγRIIB; Fc γRIIB; Fcγ RIIB; Fc γ RIIB
- Protein Length
- Thr40-Arg217
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse Fc gamma RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RIIB is greater than 90% as determined by SEC-HPLC.
OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 μM as determined in SPR assay (Biacore T200).
Loaded OKT3 on ProA-Biosensor can bind Mouse Fc gamma RIIB,His Tag with an affinity constant of 1.99 µM as determined in BLI assay (Gator).
Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
References
- Engelhardt W, Geerds C, Frey, Jürgen. DISTRIBUTION, INDUCIBILITY AND BIOLOGICAL FUNCTION OF THE CLONED AND EXPRESSED HUMAN BETA-FC RECEPTOR-II[J]. European Journal of Immunology, 2010, 20(6):1367-1377.
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