Mouse CD59a Protein
Catalog No: CD59A-MF001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
Product overview
Recombinant Mouse CD59a Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu24-Ser96 (UniProt accession: O55186).
Product Details
- Molecular Aliases
- CD59A glycoprotein;CD59a antigen; MAC-inhibitory; MAC-IP; MACIP; Membrane attack complex inhibition factor; MACIF; Protectin; CD_antigen: CD59
- Protein Length
- Leu24-Ser96
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.17 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Mouse CD59a on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD59a is greater than 95% as determined by SEC-HPLC.
Background
a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation.
References
- Sivasankar B, Donev RM, Longhi MP, Hughes TR, Davies R, Cole DS, Morgan BP, Marchbank KJ. CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens. Mol Immunol. 2007 Apr;44(11):2978-87. doi: 10.1016/j.molimm.2006.12.025. Epub 2007 Feb 12. PMID: 17296227.
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