Human VEGF R3/FLT4 Protein
Catalog No: VEGFR3-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human VEGF R3/FLT4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Tyr25-Ile776 (UniProt accession: P35916-1).
Product Details
- Molecular Aliases
- VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL;FLT4; FLT41
- Protein Length
- Tyr25-Ile776
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 111.3 kDa. Due to glycosylation, the protein migrates to 135-155 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl, 100mM Glycine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF R3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test).
Immobilized Human VEGF R3, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF-D, His Tag with the EC50 of 2.06μg/ml determined by ELISA.
Background
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
References
- Okita NT, Yamada Y, Takahari D, Hirashima Y, Matsubara J, Kato K, Hamaguchi T, Shirao K, Shimada Y, Taniguchi H, Shimoda T. Vascular endothelial growth factor receptor expression as a prognostic marker for survival in colorectal cancer. Jpn J Clin Oncol. 2009 Sep;39(9):595-600. doi: 10.1093/jjco/hyp066. Epub 2009 Jun 17. PMID: 19535387.
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