Human TNFRSF11A/Rank Protein
Catalog No: TNFRSF11A-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TNFRSF11A/Rank Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ile30-Pro212 (UniProt accession: Q9Y6Q6-1).
Product Details
- Molecular Aliases
- ODFR; Receptor activator of NF-KB; NF-KB; NFKB; CD265; TNFRSF11A; Rank; FEO; LOH18CR1; OPTB7; OSTS; PDB2; TRANCER;
- Protein Length
- Ile30-Pro212
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 46.85 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TNFRSF11A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Immobilized Human RANKL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. (QC Test)
Background
TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.
References
- von dem Knesebeck A, Felsberg J, Waha A, Hartmann W, Scheffler B, Glas M, Hammes J, Mikeska T, Yan PS, Endl E, Simon M, Reifenberger G, Pietsch T, Waha A. RANK (TNFRSF11A) is epigenetically inactivated and induces apoptosis in gliomas. Neoplasia. 2012 Jun;14(6):526-34. doi: 10.1596/neo.12360. PMID: 22787434; PMCID: PMC3394195. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.