Human TNFR2/CD120b/TNFRSF1B Protein
Catalog No: CD120B-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TNFR2/CD120b/TNFRSF1B Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu23-Asp257 (UniProt accession: P20333-1).
Product Details
- Molecular Aliases
- CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B
- Protein Length
- Leu23-Asp257
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 51.89 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TNFR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human TNF alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA (QC Test).
Immobilized Human TNFR2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFR2 Antibody, hFc Tag with the EC50 of 19ng/ml determined by ELISA.
Background
Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
References
- Vanamee, éva S, Faustman D L. TNFR2: A Novel Target for Cancer Immunotherapy[J]. Trends in Molecular Medicine, 2017:S1471491417301697. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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