Human TNFR1/CD120a/TNFRSF1A Protein
Catalog No: CD120A-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu30-Thr211 (UniProt accession: P19438-1).
Product Details
- Molecular Aliases
- TNF-R1; TNF-RI; TNFR-I; TNFR1; CD120a; TNFRSF1A; FPF; TBP1; TNF-R; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNF-R-I; TNFR1-d2;p55R; TNFR1d2
- Protein Length
- Leu30-Thr211
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human TNF alpha, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Background
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
References
- Proudfoot A, Bayliffe A, O'Kane CM, Wright T, Serone A, Bareille PJ, Brown V, Hamid UI, Chen Y, Wilson R, Cordy J, Morley P, de Wildt R, Elborn S, Hind M, Chilvers ER, Griffiths M, Summers C, McAuley DF. Novel anti-tumour necrosis factor receptor-1 (TNFR1) domain antibody prevents pulmonary inflammation in experimental acute lung injury. Thorax. 2018 Aug;73(8):723-730. doi: 10.1136/thoraxjnl-2017-210305. Epub 2018 Jan
- PMID: 29382797; PMCID: PMC6204954. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.