Human TGF-beta RII/TGFBR2 Protein
Catalog No: TGF-HF003
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TGF-beta RII/TGFBR2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ile24-Asp159 (UniProt accession: P37173-1).
Product Details
- Molecular Aliases
- TGFR2; TGFBR2; TbetaR-II; TGFβR2; TbetaR-II; TGFβR2; AAT3; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; TAAD2; TβR-II; TβR-II;TGF beta R2; TbR-II; T beta R-II
- Protein Length
- Ile24-Asp159
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TGF-beta RII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TGF-beta RII is greater than 95% as determined by SEC-HPLC.
Immobilized Human Mature TGF beta 1, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).
Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind Human Latent TGF beta 2, His Tag with an affinity constant of 2.21 μM as determined in SPR assay (Biacore T200).
Loaded Human TGF-beta RII, hFc Tag on ProA-Biosensor can bind Human Mature TGF beta 3, No Tag with an affinity constant of 0.51 nM as determined in BLI assay.
Background
The TGFBR2 gene provides instructions for making a protein called transforming growth factor-beta (TGF-β) receptor type 2. This receptor transmits signals from the cell surface into the cell through a process called signal transduction. Through this type of signaling, the environment outside the cell affects activities inside the cell such as stimulation of cell growth and division.
References
- Fricke F, Lee J, Michalak M, et al. TGFBR2-dependent alterations of exosomal cargo and functions in DNA mismatch repair-deficient HCT116 colorectal cancer cells. Cell Commun Signal. 2017;15(1):14. Published 2017 Apr 4. doi:10.1186/s12964-017-0169-y.
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