Human TACI/TNFRSF13B Protein
Catalog No: TACI-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human TACI/TNFRSF13B Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser2-Thr166 (UniProt accession: O14836-1).
Product Details
- Molecular Aliases
- CD267; TNFRSF13B; TACI; CVID; CVID2; IGAD2; RYZN; TACI
- Protein Length
- Ser2-Thr166
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 38-40 kDa and 50-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human TACI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TACI is greater than 95% as determined by SEC-HPLC.
Immobilized Human BAFF Trimer, His Tag (Cat. BAF-HM112) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human APRIL (Trimer), His Avi tag (Cat. APR-HM410B) at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TACI, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His-Flag Tag (Cat. APR-HM110) with an affinity constant of 0.76nM as determined in BLI assay.
Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag (Cat. BAF-HM112) with an affinity constant of 1.53 nM as determined in BLI assay.
Background
Transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI) is one of the receptors of B cell activating factor of the tumor necrosis factor family (BAFF) and a proliferation-inducing ligand (APRIL). TACI is a regulator in the immune responses. TACI inhibits B cell expansion and promotes the differentiation and survival of plasma cells.
References
- Zhang Y, Li J, Zhang YM, Zhang XM, Tao J. Effect of TACI signaling on humoral immunity and autoimmune diseases. J Immunol Res. 2015;2015:247426. doi: 10.1155/2015/247426. Epub 2015 Mar
- PMID: 25866827; PMCID: PMC4381970. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.