Human SLAMF6/NTB-A Protein
Catalog No: SLAMF6-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human SLAMF6/NTB-A Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln22-Met226 (UniProt accession: Q96DU3-1).
Product Details
- Molecular Aliases
- ANK-T-B-antigen; NTB-A; CD352; SLAMF6; KALI; Ly108; NK-T-B-antigen; KALIb; SF2000
- Protein Length
- Gln22-Met226
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human SLAMF6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SLAMF6 is greater than 90% as determined by SEC-HPLC.
Immobilized Human SLAMF6, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-SLAMF6 Antibody, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA.
Background
SLAMF6 (signaling lymphocyte activation molecule 6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules. It is known to be widely and exclusively expressed on hematopoietic cells. The SLAMF6 intracellular portion is characterized by two ITSMs that act as binding sites for adaptor molecules such as SAP and EAT-2.
References
- Dragovich MA, Adam K, Strazza M, Tocheva AS, Peled M, Mor A. SLAMF6 clustering is required to augment T cell activation. PLoS One. 2019 Jun 14;14(6):e0218109. doi: 10.1371/journal.pone.0218109. PMID: 31199820; PMCID: PMC6568412.
- Yigit B, Wang N, Ten Hacken E, Chen SS, Bhan AK, Suarez-Fueyo A, Katsuyama E, Tsokos GC, Chiorazzi N, Wu CJ, Burger JA, Herzog RW, Engel P, Terhorst C. SLAMF6 as a Regulator of Exhausted CD8+ T Cells in Cancer. Cancer Immunol Res. 2019 Sep;7(9):1485-1496. doi: 10.1158/2326-6066.CIR-18-0664. Epub 2019 Jul
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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