Human SIRP alpha/CD172a Protein
Catalog No: SIRPA-HP001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human SIRP alpha/CD172a Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Glu31-Arg370 (UniProt accession: P78324-1).
Product Details
- Molecular Aliases
- CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA;
- Protein Length
- Glu31-Arg370
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SIRP alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human SIRP alpha, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-SIRP alpha Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 73.46ng/ml.
Human SIRP alpha, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200).
Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200).
Background
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
References
- Weiskopf K, Ring A M, Ho C C M, et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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